Real-time Polymerase Chain Reaction:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
SYBR Green Assay:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Reverse Transcription Polymerase Chain Reaction:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Mutagenesis:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Enzyme-linked Immunosorbent Assay:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
dsDNA Assay:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Formalin-fixed Paraffin-Embedded:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Extraction:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Ubiquitin Proteomics:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Conjugation Assay:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
RNA Sequencing:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Quantitative RT-PCR:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
Recombinant:Article Title: Ubiquitination-directed cytosolic DNA degradation governs cGAS-STING-mediated immune response to DNA damage.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 2×TSINGKE Master qPCR Mix (SYBR Green I) TSINGKE Cat# TSE201 PrimeScriptTM RT PCR Kit Takara Cat# RR014A KOD Plus Mutagenesis Kit Toyobo Cat# SMK101 Mounting Medium with DAPI - Aqueous, Fluoroshield Abcam Cat# ab104139 M-PER buffer Thermo Fisher Cat# 78501 cGAMP ELISA kit Cayman Chemical Cat# 501700 SpectraMax Quant AccuClear Nano dsDNA Assay Kit Molecular Devices Cat# R8364 TRIzol Invitrogen Cat# 15596026CN QIAamp DNA FFPE Tissue kit QIAGEN Cat# 56404 ECL reagent BioRad Cat# 1705061 2 × Hot Start Taq Master Mix Novoprotein Cat# E028-02A GeneJET Extraction kit Fermentas Cat# K0692 E2 Select Ubiquitin Conjugation Kit Yeasen Bio Cat# 20440ES10 Deposited data The Cancer Genome Atlas (TCGA) pan cancer dataset Cancer Genome Atlas Research, 2015 Cancer Genome Atlas Research Network Raw RNA seq data This paper GEO: PRJNA1270440 and PRJNA1267489 Raw data and images This paper https://doi.org/10.17632/vy2cbjn3my.1 Experimental models: cell lines Human: HEK293T ATCC CRL-11268 Human: PC-3 ATCC CRL-1435 Human: C4-2 ATCC HTB-96 Human: SW48 ATCC CCL-231 Human: A375 ATCC CRL-1619 Human: SiHa ATCC HTB-35 Human: C-33A ATCC HTB-31 Mouse: RM1 ATCC CBP60880 Mouse: Pten/Rb1 DK This paper N/A Oligonucleotides Primers for RT-qPCR This paper see Table S2 sgRNAs This paper see Table S2 Recombinant DNA pcDNA3.1(+)-Flag-TREX1 Geneppl, China Cat# PPL01544-2a pCMV3-SPOP-Myc From Dr. Jian Ma N/A pCMV3-SPOP F102C -Myc From Dr. Jian Ma N/A pCMV3-SPOP F133V -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔMATH -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBTB -Myc From Dr. Jian Ma N/A pCMV3-SPOP ΔBACK -Myc From Dr. Jian Ma N/A pTsin-CMV-SPOP-HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F102C -HA From Dr. Yizeng Fan N/A pTsin-CMV-SPOP F133V -HA From Dr. Yizeng Fan N/A pCMV-Flag-USP7 From Dr. Yizeng Fan N/A Flag-TREX1 ΔSBC This paper N/A Flag-USP7 C223A This paper N/A plenti-hygro-GFP-TREX1 This paper N/A plenti-hygro-GFP-TREX1ΔSBC This paper N/A (Continued on next page) Cancer Cell 44, 306–320.e1–e7, February 9, 2026 e3
|
ATCC is a verified supplier 
ATCC manufactures this product 